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Freegrass

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Precision Fermentation
« on: May 31, 2023, 10:10:59 PM »
In this video that Sigmetnow posted, I heard talk about "precision fermentation" (or industrial fermentation as it is called on Wikipedia) and I had no idea what that was. So I looked it up, and I am blown away. So much so that I believe it definitely deserves its own thread.

If there was ever a planet saving technology, this is way up there. I already knew about lab grown meat, but precision fermentation is so much more than that. And it is happening at a much faster pace than I could ever have imagined...

Before I started this new thread, I searched for precision fermentation on the forum, and there was only one mention of this technology in an article that also Sigmetnow posted on March 12 this year. I completely missed that debate. Maybe it's good if that discussion gets moved over here Kassy? Because this technology is going to be huge.

To start it off, here's an excellent video about precision fermentation from about 2 years ago. The Flexitarian Times has 5 videos on their channel, and they're all worth your time. I learned a lot from them. They are a good introduction to the technology.

The complete RethinkX report can be downloaded in PDF at the bottom.

« Last Edit: June 01, 2023, 10:51:35 AM by Freegrass »
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Freegrass

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Re: Precision Fermentation
« Reply #1 on: May 31, 2023, 10:18:47 PM »
Brewing up a new business: AB InBev surprises with ambitions for animal-free proteins

https://biovox.eu/brewing-up-a-new-business-ab-inbev-surprises-with-ambitions-for-animal-free-proteins/

Belgian-American AB InBev, the world’s largest brewer, has set up a separate R&D division called BioBrew to develop animal-free proteins using precision fermentation. Quite a number of companies are already focused on producing alternative proteins, which don’t cause harm to animals and are more sustainable, but they generally lack the capacity to produce them on a commercial level. This is one of the things that sets BioBrew apart, thanks to AB InBev’s knowhow of using yeast fermentation on a large scale to make beer.

For multiple reasons, interest in the production of non-animal proteins is on the rise. Among these incentives are the lower ecological impact, contrasted with the huge greenhouse gas emissions and deforestation associated with industrial livestock farming, and the fact that these production processes don’t cause harm to animals. With the rapid growth of the world population leading to a need for more efficient food systems, industry has been searching for a way to produce proteins as a commercial scale.

“There are many small biotech companies which are developing such alternative proteins, but almost all of them lack the expertise to scale up their processes,” said David De Schutter, the head of  AB InBev’s global innovation center Gitec, to Belgian newspaper De Standaard. “We however do possess this knowhow. As the world’s largest brewer, we know perfectly how to efficiently convert yeast in huge amounts of beer. In our brewery in Leuven, we have fermentation tanks of 400,000 liters.” The Gitec center, located across the AB InBev headquarters in Leuven, employs 122 scientists to develop new products and better brewing processes.

Programming yeasts to deliver proteins

Just as AB InBev uses yeasts to make beer, it now wants to put those single-celled microorganism to work to ‘brew’ proteins with the BioBrew project. “Yeasts are like small factories which can duplicate themselves quickly and convert substances into other ones,” explained De Schutter. Yeast cells are essential for making not only beer, but also for example wine, cheese, and bread. AB InBev itself possesses a collection of around 1,700 yeast strains, which are crucial for the flavors of its beers.

During a beer-brewing process, yeast is programmed to convert sugar into alcohol, but the BioBrew experts will program them in such a way that they deliver proteins instead. This kind of programming of microorganisms to create very specific products is called ‘precision fermentation’.

Once they are ready for their precise task, the yeast cells will be put in large fermentation vessels and fed with energy-rich sugar to jump-start the fermentation process. According to AB Inbev, one full BioBrew fermentation tank can contain food equivalent to 5,000 cows or 300,000 chickens. At the end of the process, the result can be purified until only protein powder is left, which can then be used as an ingredient by the food industry.

Plans for large production units

AB InBev started with the BioBrew project in 2019 and it quickly turned out to be a success. Part of this success is also due to collaborations with research centers in Belgium, such as the Flemish life sciences institute VIB, the spearhead cluster for the agri-food industry Flanders’ FOOD, and different Belgian universities.

The Belgian prime minister Alexander De Croo recently paid a visit to Gitec to get to know BioBrew. He was impressed by AB InBev’s ventures in the domain of alternative proteins. “Belgium is not just a country of beers; our researchers are also leaders in biotech,” said De Croo. “It’s impressive to see how one of our Belgian brewers is combining those assets and thus getting ahead of the competition.”

Apart from the research taking place in Leuven, AB InBev has together with a partner also set up a first pilot plant for proteins in Germany. Later, the plan is to build separate, large production units. David De Schutter said to De Standaard that he couldn’t share much about the timing and location of these installations, only that there will probably be four of them, and that the units would also be open to smaller companies developing animal-free proteins.
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Freegrass

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Re: Precision Fermentation
« Reply #2 on: June 01, 2023, 03:35:05 AM »
People are really scared that the entire dairy industry will collapse by 2030. Amazing... No more cow milk... Who would have thought?

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Freegrass

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Re: Precision Fermentation
« Reply #3 on: June 01, 2023, 04:32:22 AM »
I was wondering if it would ever be possible to create vegetable oils with PF, and so I went to chatgpt, and had this conversation with it. It seems like this may be possible in the future. And if that's true, we probably will be able to get rid of palm oil plantations too...

I know, I know... I'm going way too fast. But PF really is an amazing technology it seems. I can't believe we missed this... Still a lot to learn about it of course, but that's why we have this thread now. I can't wait for all your input. I really want to know how far they can push this technology...

User (me)
Can vegetable oil be made with Precision Fermentation?

ChatGPT
As of my knowledge cutoff in September 2021, vegetable oil is typically produced through processes such as pressing or solvent extraction from oil-rich plants or seeds. Precision fermentation, on the other hand, is a technology that involves using genetically modified organisms (GMOs) to produce specific compounds through fermentation.

While precision fermentation has been used to produce a range of products, including proteins and flavors, it is not commonly used to produce vegetable oils. The production of vegetable oils involves extracting fats from plant sources, which is different from the fermentation process used in precision fermentation.

However, it's worth noting that the field of biotechnology is rapidly advancing, and new techniques and applications may have emerged since my last update. Therefore, it's always a good idea to consult the latest scientific literature or industry sources for the most up-to-date information on the use of precision fermentation in vegetable oil production.

User
is vegetable oil a protein?

ChatGPT
No, vegetable oil is not a protein. Vegetable oil is primarily composed of fats, specifically triglycerides, which are a type of lipid. Proteins, on the other hand, are complex macromolecules composed of amino acids and are involved in various biological functions, such as providing structure to cells, catalyzing biochemical reactions, and facilitating cellular communication.

Vegetable oils, including oils derived from plants such as soybean, canola, sunflower, and olive, contain predominantly fatty acids, which are a type of lipid. These fatty acids provide a concentrated source of energy and are essential for various physiological processes in the body. However, they do not contain proteins.

User
Precision Fermentation can make milk, and milk contains fat. So how do you make those fats with Precision Fermentation?

ChatGPT
Precision fermentation can indeed be used to produce milk proteins through the fermentation of genetically modified microorganisms. However, the production of milk fats through precision fermentation is a more complex process.

Milk fats are composed of various types of fatty acids, including saturated, monounsaturated, and polyunsaturated fats. To produce these fats using precision fermentation, researchers would need to identify and modify microorganisms capable of synthesizing the desired fatty acids. This would involve introducing genes encoding the enzymes responsible for fatty acid synthesis into the microorganisms.

The modified microorganisms would then be cultured and provided with appropriate nutrients to support their growth and fatty acid production. The fermentation process would need to be carefully controlled to optimize the production of specific fatty acids and achieve the desired composition of milk fats.

While precision fermentation has shown promise in producing various compounds, including proteins, flavors, and other ingredients, the production of complex fats like those found in milk is still an ongoing area of research and development. It may take time before precision fermentation becomes a commercially viable method for producing milk fats at scale.

User
If they would be able to create those fatty acids, would the next step not be to create oils from them?

ChatGPT
Yes, if researchers are able to produce specific fatty acids using precision fermentation, the next step would be to convert those fatty acids into oils. Vegetable oils, such as soybean oil, canola oil, or sunflower oil, are primarily composed of triglycerides, which are esters formed from glycerol and fatty acids.

To create oils from the produced fatty acids, a process called esterification or transesterification would be employed. In this process, the fatty acids are combined with glycerol to form triglycerides. This can be done through chemical reactions or enzymatic processes.

Once the triglycerides are formed, they can be further processed, refined, and purified to obtain the desired vegetable oil product. This typically involves steps such as filtration, degumming, neutralization, bleaching, and deodorization.

It's important to note that while precision fermentation has the potential to produce specific fatty acids, the conversion of those fatty acids into oils would involve additional steps beyond the fermentation process. These subsequent processing steps would be necessary to obtain the final vegetable oil product.
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Freegrass

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Re: Precision Fermentation
« Reply #4 on: June 01, 2023, 04:39:36 AM »
What are the dangers of Precision Fermentation?

ChatGPT
Precision fermentation, like any emerging technology, presents both opportunities and challenges. While it has the potential for various benefits, there are also potential risks and concerns associated with its use. Some of the dangers and considerations of precision fermentation include:

1. Environmental impact: Precision fermentation may involve the use of genetically modified organisms (GMOs), and the release of such organisms into the environment could have unintended ecological consequences. It is crucial to assess and manage potential risks to ecosystems, biodiversity, and the spread of modified genes to wild populations.

2. Health and safety concerns: The safety of products derived from precision fermentation, such as novel proteins or ingredients, should be thoroughly evaluated to ensure they do not pose risks to human health, including allergies or toxicological effects. Proper regulatory oversight is essential to assess and manage potential risks to consumers.

3. Regulatory challenges: The development and commercialization of precision fermentation products may face regulatory hurdles, as different countries have varying regulations regarding GMOs and novel ingredients. Ensuring appropriate oversight, labeling, and safety assessments can be complex and require clear regulatory frameworks.

4. Ethical considerations: The use of precision fermentation may raise ethical concerns related to genetically modifying organisms and altering natural systems. Questions regarding the impact on traditional agriculture, farmer livelihoods, and the potential concentration of power in the hands of a few large corporations may arise.

5. Unintended consequences: Genetic modifications and engineering can have unpredictable effects, and unintended consequences may arise during precision fermentation processes. Thorough risk assessments and monitoring are necessary to identify and mitigate any unforeseen impacts.

It's important to note that these risks can be managed and mitigated through robust scientific research, responsible development, stringent regulatory oversight, and transparent communication. As the field progresses, it is essential to address the potential dangers while harnessing the benefits of precision fermentation.
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Freegrass

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Re: Precision Fermentation
« Reply #5 on: June 01, 2023, 05:18:28 AM »
Be cautious of the techno fix

https://www.foodethicscouncil.org/opinion/be_cautious_of_techno_fix

Technologies – applied properly – have an important role to play in sustainable food systems. However, Patti Whaley, Trustee of the Food Ethics Council, argues we should be wary of hoping that techno fixes will make our existing ethical dilemmas go away. It’s more likely that they will solve some, create some new ones, and leave some unchanged.

At first glance, the recent RethinkX report on the future of food and agriculture might sound like good news. According to their predictions, replacing intensive livestock farming with precision fermentation of protein will largely do away with a host of food sector “issues”, including poor nutrition, high or unstable food prices, food-borne disease, high environmental footprint, biodiversity loss and poor animal welfare. I’m not about to give odds on whether their predictions will come true; after all, lab-based meat has been “just over the horizon” for some time now. But it’s worth giving some thought, with our Food Ethics hat on, to why technological solutions are so seductive, and whether the lure of an easy technological fix hides some deeper ethical issues.

Some background: RethinkX is an independent thinktank that analyses and forecasts the speed and scale of technology-driven disruption and its implications across society. They have produced two reports so far – one on disruption in the transportation sector, and this latest one on the food sector. Both reports predict widespread technologically-driven disruption within the next ten years. These disruptions will cause an initial loss of jobs and economic value, but will, according to RethinkX, radically reduce both the economic and the environmental cost of food and transport. If managed properly, they say, the food sector disruption will also decentralise and redistribute power within the food sector, shifting us away from a system dominated by large corporate players, capital-intensive production, and haggling over intellectual property (‘IP’) and trade agreements. I’m not here to endorse or condemn the RethinkX report – but I am interested in the questions it raises about our approach to problems in the food system.

RethinkX’s argument is presented in a largely objective, non-judgemental way, although there is clearly an underlying view that the predicted benefits of this disruption ought to be widely distributed for the public good, rather than closely protected for corporate profit. In particular, they highlight the importance of how intellectual property will be licensed: we should “Allow companies to patent production methods but not life, genes, or molecules – IP regimes should be process-focused rather than output-focused.” They also argue that the forces of decentralisation and competition will tend to minimise barriers and maximise broad public benefit.

On the surface – so far, so good. And it’s not surprising, given the challenges of world hunger and environmental degradation, that a technological path out of our current situation looks so attractive. But if we think more deeply, there are important questions here about wellbeing, power, winners and losers, what choices we make, and why we keep hoping that technology will save us from ourselves. To list just a few of these:

Would an abundance of cheap protein improve our wellbeing?
For most of us, no. Most people on Western-based diets do not need more protein in their diets – we already eat too much, when we should be eating more vegetables and whole grains. For too long now, we have equated a high-protein diet with affluence; in developing countries such as China, a rise in the standard of living has led to greater consumption of meat, in spite of evidence that a Mediterranean or largely plant-based diet can be better for us. Fresh fruit and vegetables already look relatively expensive, particularly when compared to highly-processed sugars, fats and carbohydrates; a big drop in the relative cost of protein might make fruits, vegetables and whole grains look even less affordable. Although there are undoubtedly poor areas of the world where additional protein would improve overall diets, for most of us, a ready supply of cheap protein could result in a less diverse and healthy diet.

Would a different technology lead us to a more decentralised, open-source, public-benefit food system?
Not necessarily. Food production certainly used to be a highly-decentralised and localised activity where seeds, breeds and farming know-how were shared more openly. But the forces of corporate capitalism seem to have pushed us towards ever greater concentration of power, and companies have been adept at protecting their interests through intellectual property law and international trade agreements. Countries such as the United States have used their trade-negotiating power to dump their excess grain reserves on poorer countries, for example, undercutting local markets and driving local food producers into bankruptcy. A production technology that requires less capital may partially offset this trend, but it’s unlikely to slow it down for long. I’d agree with RethinkX that an IP regime that protected processes, but not specific proteins, would be preferable; but achieving such a regime would run counter to the IP trends of the last several decades.

Do we need a new technology to solve the environmental problems of our food system?
Not necessarily. Much of the high environmental cost of our food system could be eliminated without any new technology at all. The benefits proposed by RethinkX – a rebuilding of fish stocks, less use of antibiotics and growth hormones, lower rates of food-based illness, a drop in our use of petrol-based products, a reduction in animal suffering – could all be ours now, if we were willing to shift towards more plant-based diets and eat “less but better” meat.

Perhaps most importantly: Do we need a new technology to feed the world’s billions?
No. Technology can certainly help, but we could feed everyone now if we wanted to — perhaps not a Western high-protein diet, but a perfectly adequate and reasonably varied one, and arguably a healthier one. The problem of world hunger, or even UK hunger, is not a problem of production; it’s a problem of poor distribution, and the prioritising of private profit over public goods.

The reason that laboratory protein looks so appealing is that it promises that we can continue to eat in the ways we are accustomed to, without the associated costs. By disassociating protein production from livestock farming, we can “have our [burger] and eat it too”. That promise may or may not come true, but it would still leave us with some important choices to make. As societies, we would still need to choose to share the benefits of technological innovation with our poorer neighbours, rather than using them to retain the economic upper hand; and to use the resources freed up by disruption for public good rather than private profit. We would still need to choose to treat our food producers and food servers fairly and with dignity. As individuals, we would still need to choose varied and healthy diets, not just cheap ones. And for those of us who love to cook and eat good food, and who are fortunate enough to be able to do so, we could still choose to treat food as a source of creativity, comfort and joy, rather than a cheap and convenient commodity.

The problem with future technology promises is that they can tempt us to put off urgent reforms to our food system. Maybe, just maybe, some silver bullet will come along that will resolve our dilemmas for us, but relying on that hope would be a mistake. We can make better choices right away.

Guest blog from Patti Whaley, Trustee of the Food Ethics Council



What Consumers Should Ask About Precision Fermentation

https://www.forbes.com/sites/errolschweizer/2022/03/02/what-should-consumers-be-asking-about-precision-fermentation/

Even Forbes has a lot of questions it seems. It's a long article, and I need to get some sleep now. So I'm going to finish reading this tomorrow. But it started good, and has a lot of links in it.

Enjoy!
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kassy

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Re: Precision Fermentation
« Reply #6 on: June 01, 2023, 09:25:49 AM »
Quote
Would an abundance of cheap protein improve our wellbeing?
For most of us, no. Most people on Western-based diets do not need more protein in their diets – we already eat too much, when we should be eating more vegetables and whole grains.

There are huge gains to be made if we would eat the recommended amount of red meat instead of the current intake. This is quite a strong effect which even reduces some forms of river pollution because excess protein gets execrated.

Anyway lots happening on the PF front so we shall collect that here.
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Sigmetnow

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Re: Precision Fermentation
« Reply #7 on: June 01, 2023, 03:37:04 PM »
Here’s Tony Seba’s discussion:
 
The Great Transformation [Part 4] - The #Disruption of #Food & #Agriculture
Nov 15, 2022.  17 min.
➡️
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Freegrass

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Re: Precision Fermentation
« Reply #8 on: June 01, 2023, 06:51:15 PM »
Here’s Tony Seba’s discussion:

The Great Transformation [Part 4] - The #Disruption of #Food & #Agriculture
Nov 15, 2022.  17 min.
Thanks for that link, Sigmetnow. Did you already post the transportation video on the EV thread? Definitely worth it! I'm on part 3 now, Energy. This is a truly amazing guy, and he makes perfect sense... The next ten years are going to be truly exciting, and game changing.

I recommend everyone to watch his 5 videos! They will change your life... Suddenly, I'm not so pessimistic anymore. As long as we can take out some CO2 again, the future is looking a lot better now... 2030!
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Re: Precision Fermentation
« Reply #9 on: June 01, 2023, 07:15:16 PM »
There is a Tony Seba thread, although it has not been active since last fall.

https://forum.arctic-sea-ice.net/index.php/topic,2073.msg352419.html#msg352419
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Freegrass

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Re: Precision Fermentation
« Reply #10 on: June 01, 2023, 10:02:22 PM »
There is a Tony Seba thread, although it has not been active since last fall.

https://forum.arctic-sea-ice.net/index.php/topic,2073.msg352419.html#msg352419
Ah, ok. Thanks! One more thread I missed... I'll check out the comments there. He's my new favorite guy on the planet right now... Hope he has a chat with Michael Liebreich soon, but it looks like they're not best friends...

https://twitter.com/MLiebreich/status/1129845889303027713?s=20
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Re: Precision Fermentation
« Reply #11 on: July 27, 2023, 11:47:06 AM »
A great new documentary on (dairy) farming in New Zealand, and a very good argument why we need precision fermentation ASAP.

Not sure how long it'll be online for free, so hurry up watching it.
(ignore the preview. Just click the "learn more" button to open the video)

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Re: Precision Fermentation
« Reply #12 on: July 27, 2023, 12:04:20 PM »
Disrupting dairy with precision fermentation: ‘By 2035, industrial cattle farming will be obsolete’
03-Feb-2020 By Flora Southey

The imminent collapse of dairy and cattle industries has been forecast by think tank RethinkX, with precision fermentation protein set to ‘outperform the cow’.

Read the whole article here...
When factual science is in conflict with our beliefs or traditions, we cuddle up in our own delusional fantasy where everything starts making sense again.